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Daniel Dunaief

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From left, Mikala Egeblad and Xue-Yan He. Photo from Constance Brukin

By Daniel Dunaief

They both have left Cold Spring Harbor Laboratory, but the innovative research they did on Long Island and that they continue to do, is leaving its mark.

From left, Mikala Egeblad and Xue-Yan He at the American Association for Cancer Research (AACR) annual meeting in New Orleans, Louisiana in 2022. Photo from Xue-Yan He

When Xue-Yan He was a postdoctoral researcher in the lab of Mikala Egeblad, who was Associate Professor at CSHL, the tandem, along with collaborators, performed innovative research on mice to examine how stress affected the recurrence and spread of cancer in a mouse model.

In a paper published in late February in the journal Cancer Cell, He, who is currently Assistant Professor of Cell Biology & Physiology at Washington University School of Medicine in St. Louis, discovered that stress-induced neutrophil extracellular traps (NETs), which typically trap and kill bacteria, trigger the spread of cancer.

“The purpose of our study is to find out what stress does to the body” of an animal model of cancer, said He.

The data in mice demonstrated that targeting NETs in stressed animals significantly reduced the risk for metastases, He explained, suggesting that reducing stress should help cancer treatment and prevention. The researchers speculate that drugs preventing NET formation can be developed and used as new treatments to slow or stop cancer’s spread.

To be sure, this finding, which is encouraging and has generated interest among cancer scientists and neurobiologists, involved a mouse model. Any potential application of this research to the diagnosis and treatment of people will take considerably more effort.

“I want to stress that the evidence for the link between stress, NETs, and cancer is from mouse studies,” Egeblad explained. “We will need to design human studies to know for sure whether the link also exists for humans.”

Still, Egeblad hopes that eventually reducing stress or targeting NETs could be options to prevent metastatic recurrence in cancer survivors. “One major challenge is that a cancer diagnosis by itself is incredibly stressful,” she explained. The results of these experiments have attracted considerable attention in the scientific community, where “there is a lot more to learn!” 

Three part confirmation

When she was a postdoctoral researcher, He removed neutrophils from the mice using antibodies. Neutrophils, which are cells in the immune system, produce the NETs when they are triggered by the glucocorticoid stress hormone.

She also injected an enzyme called DNAse to destroy NETs in the test mice. The former CSHL postdoctoral researcher also used genetically engineered mice that didn’t respond to glucocorticoids.

With these approaches, the test mice developed metastasis at a much lower rate than those that had intact NETs. In addition, chronically stressed mice who didn’t have cancer had NETs that modified their lung tissue.

“Stress is doing something to prepare the organs for metastasis,” said He.

Linda Van Aelst, CSHL Professor and a collaborator on the study, suggested that this work validates efforts to approach mental health in the context of cancer.

“Reducing stress should be a component of cancer treatment and prevention,” Van Aelst said in a statement.

After He removed the primary tumor in the mouse models, the stressed mice developed metastatic cancer at a four-fold higher rate than the mice who weren’t stressed but who also previously had cancer.

The CSHL scientists primarily studied breast cancer for this work.

He appreciated the help and support from her colleagues at CSHL. “To really understand the mechanism” involved in the connection between stress and cancer, “you need a mouse model in the lab, an expert in neuroscience and an expert in the cancer field,” she said.

As a neuroscientist, Van Aelst offered suggestions and comments and helped He conduct behavioral tests to determine a mouse’s stress level. The work for this project formed the focus ofHe’s postdoctoral research, which started in 2016 and ended in 2023.

The link between stress and cancer is receiving increasing attention in the scientific community and has attracted attention on social media, He said.

CSHL “provided a great environment to perform all these experiments,” said He. The numerous meetings CSHL hosts and the willingness of principal investigators across departments made the lab “one of the best places” for a postdoctoral scientist.

“If you need anything from a neural perspective or a technical perspective, you can always find a collaborator” at CSHL, He added.

Born and raised in Nanjing, China, He enjoyed living on Long Island, visiting vineyards and trying to explore every state park. In the harbor, He caught blue crabs while her husband Chen Chen, who was a postdoctoral researcher at CSHL in the lab of Camila dos Santos, went fly fishing at Jones Beach.

In her current research, where she manages a lab that includes a senior scientist, a postdoctoral researcher and an undergraduate, He is extending the work she did at CSHL to colorectal cancer, where she is also analyzing how stress affects the spread of cancer.

“When you’re stressed, you can develop gastrointestinal problems, which is why I wanted to switch from breast cancer to colorectal cancer,” she said.

Extensions of the work

As for context for the research at CSHL, Egeblad wrote that doctors treating patients where the known risk of recurrence is high might use NETs in the blood as a biomarker.

The scientists think cancers that tend to metastasize to the liver, lung or spleen are the strongest candidates to determine the effect of NETs and stress on cancer.

“We have not seen any effects of targeting NETs for metastasis to the bone or the brain in our mouse model and similarly, the studies that have linked NETs to metastasis in human patients have mostly been cancer that has spread to the liver or the lung,” Egeblad said.

Egeblad appreciated the “fantastic job” He did on the work and described her former researcher as being “fearless.”

“She found that stress increased metastasis early in her project but it was a lot of work to discover it was the NETs that were responsible and to conduct studies to ensure that the results were applicable to different types of cancer,” Egeblad explained.

While the two researchers have gone to different institutions and are leading other lab efforts, Egeblad said she’d be happy to collaborate with her former student, who shares the same sense of humor.

Egeblad recalled how He ended her talks by telling the audience that her results showed that Egeblad should give her a “long vacation.”

“I think indeed that she has deserved one after all this work!” Egeblad offered.

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Pixabay photo

By Daniel Dunaief

Daniel Dunaief

I was in between that state when I’m focused on how tired I am at the gym and when the endorphins kick in, enabling my body to push harder and for longer in the interests of physical fitness and mental health.

When my cell phone rang, I wasn’t sure whether to pick it up. I’ve been getting numerous annoying robocalls from pseudo-people who want to sell me something I don’t want or need. When I ask them to take my name off their lists, they hang up and someone else from the same organization calls me back the next day.

Unless I recognize the number or am expecting an important call, I tend to let voicemail pick up while I disconnect from everything but the rhythm of checking the number of calories I’ve burned and the distance I’ve traveled during each five minute segment on the elliptical machine.

This time, however, the name looked vaguely familiar, so I stopped moving, took out the airpods that don’t work too well and picked up the phone.

“Hi, this is Dan,” I said, trying to control my breathing.

“Who is this?” the person asked.

“Dan, why, who is this?” I thought, as I considered disconnecting and returning to my routine.

“I have a number that my wife wrote down on my desk and I wanted to know who this was,” he said.

That’s when it hit me. The name was familiar because I had written a story a few weeks ago and had reached out to the couple for a comment.

He understood my explanation and asked if I were related to several other Dunaiefs he knew.

“Yup, that’s my mother,” I said proudly, awaiting words of appreciation and praise for what she’s done since she started the newspapers over 47 years ago and become a visible presence in the community.

“And Ivan?” he asked, “That’s your father?”

“Indeed,” I said.

“Well, I knew him many years ago,” he offered. “We worked together.”

I nodded and looked around the room to see if anyone were waiting to use the elliptical machine. Fortunately, no one was hovering.

“So, how is he?” he asked.

“Excuse me?” I replied, not sure I heard him correctly.

“How is Ivan doing?”

I hadn’t been asked this question in decades.

“He died in 1987,” I said, flatly.

“Oh,” he said, “1987?”

“Yes,” I said.

“Well, we all have to go sometime,” he offered. “Some sooner than others, I suppose.”

After we ended the call, I resumed my exercise. That seemed like a surprisingly flippant thing to say. The older, current version of me was annoyed, while the younger version felt vulnerable.

Once I built up a solid sweat, a broad smile filled my face, leading at least one person to ponder why I looked unusually pleased during physical exertion.

While I knew the man was processing the not-so-new news, I also decided that the person who would have taken particular delight in this slightly absurd conversation was my father.

With my legs pumping away, I shared a laugh with my father, who could make me smile no matter how frustrated or annoyed I was as a teenager.

Over time, I have enjoyed any number of opportunities to connect with people I’ve had the privilege of knowing who have died, sometimes through dreams or by watching, hearing or experiencing something I know they’d appreciate.

Recently, after my mother-in-law died, my wife received a set of wind chimes with her mother’s name inscribed on them. Before we placed the chimes, we rarely had much wind. Now, amid a steady flow of unusual breezes that bring pleasant sounds to our backyard, my wife and I smile at each other.

If we look for it, we can take comfort in the things that help us feel connected to those we’ve lost.

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Georgios Moutsanidis, Photo by Ram Telikicherla

By Daniel Dunaief

In the best of times, water provides a serene background, offers an escape from searing summer heat, serves as a livelihood for the fishing industry, and supports a range of aquatic life that shimmers just below the surface.

In the worst of times, that same water can threaten communities that line coasts, bringing a powerful surge of destructive force that takes lives and destroys homes, buildings and infrastructure.

Recently, Georgios Moutsanidis, Stony Brook University Assistant Professor in the department of Civil Engineering, received a $500,000, five-year Faculty Early Career Development grant from the National Science Foundation to conduct research that could increase the resilience of coastal structures.

Rigoberto Burgueño, who is the chairman of the Civil Engineering department and who helped recruit Moutsanidis to Stony Brook in 2020, is pleased with the recognition from the NSF.

It is “one of the highest achievements for an individual investigator in terms of their potential as future leaders in their field and future mentors and teachers,” Burgueño said. The prestige from the award “will provide opportunities and bigger audiences to communicate his findings and his work.”

Amid climate change, the need for efforts to improve resilience from a range of water-driven forces increases, as rising sea levels encroach on coastlines and stronger storms driven by higher ocean temperatures threaten buildings and infrastructure.

“What we are trying to do with this project is to develop state-of-the-art computational tools that engineers and other researchers will use to incorporate in their work and study the resilience of structures against extreme hydrodynamic events” such as storm surges and tsunamis, said Moutsanidis.

Engineers, city planners and builders have used what Moutsanidis described as mostly outdated empirical models to test the resilience of structures. Moutsanidis, however, hopes to enhance those models by taking a physics-based numerical approach to understanding the damage a surge of water could do to various structures.

Moutsanidis is using established and well-known equations. He will contribute to solving them more accurately and efficiently.

Other models “could simulate water hitting a structure, but they were unable to capture the detailed response of the structure, with cracks, fractures, fragmentation and collapse,” Moutsanidis said. He hopes the new computational methods he will develop will predict the type and extent of damage more accurately.

The model he plans to create, with the help of graduate students he will hire who will use new high-performance computers he expects to use the funds to purchase, can address site-specific features of an area that would affect the likely speed, amount and force of any water surge.

Burgueño described Moutsanidis as being “at the forefront of very advanced computational simulations that take into account interactions of water with a structure.”  

By generating better estimates of the actual loads imposed on a structure, “we will be better prepared as engineers to either strengthen existing structures or to design future ones better,” Burgueño said.

Checking his work

While the information he’s using to construct these models relies on physics and deploys established equations, the Stony Brook Assistant Professor and his students will perform verification and validation. They will compare their results with existing experimental data and other computational approaches.

In addition, Moutsanidis’s lab will conduct experiments in a flume, which is a water tank in which he can vary the amount and speed of water approaching models of coastal communities. With a high-speed camera, he can evaluate how these simulated structures respond. In buildings that might collapse or fracture, he can test a slightly different fortified design, run a similar analysis and determine if the modifications led to a better outcome.

At this point, Moutsanidis has completed a proof of concept article in the journal Engineering with Computers, where he demonstrated the idea and the equations he’s solving. He hopes to produce a useful package that engineers and the public can use within the next four to five years, which he will release through an open source platform such as GitHub.

Moutsanidis is “very eager to start this work” as storm surges, flooding and tsunamis threaten coastal communities every year.

Larger context and other projects

In the bigger picture, Moutsanidis seeks to use computational methods and software to solve problems of engineering driven by physics. He has also worked in the aerospace community, studying the interaction of solids with hypersonic flow.

The goal is to “design more efficient aerospace vessels” that can withstand high temperatures and pressure as they travel five times the speed of sound or more, he said. The temperature is so high that the air undergoes a chemical reaction.

Moutsanidis has also worked with the impact of blast waves on structures, simulating the response to the shock wave or blast.

The goal is to make structures “more resilient or resistant to extreme events” such as a terrorist attack or an accident that triggers an explosion.

From Karditsa to Queens

Born and raised in a small town in the center of Greece called Karditsa, Moutsanidis is the son of two engineers. “In my early childhood, I was influenced by them, but I chose a different engineering path,” he said.

Moutsanidis, who completed postdoctoral research at Brown University before joining Stony Brook, lives in Queens. 

Moutsanidis is impressed with the students at Stony Brook, whom he described as “very engaged.” As for his work, he explained that his field is “quite competitive” and he was surprised and pleased to receive this award.

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The Whaling Museum & Education Center of Cold Spring Harbor, in partnership with TBR News Media of Setauket, has announced the launch of a Sea Glass Fiction Contest. This exciting competition invites students in grades 3 to 12 from Nassau and Suffolk counties to unleash their creativity by crafting stories inspired by a piece of sea glass.

Students are asked to craft a story inspired by the above sea glass fragment.

The contest, which kicked off this month, challenges participants to imagine a unique journey for the selected sea glass fragment chosen by The Whaling Museum and to weave a captivating tale around it. The winning stories will have the chance to be read at the museum’s annual Sea Glass Festival, a prestigious event celebrating sea glass and its significance in maritime history. 

“Sea glass has a way of capturing the imagination, and we can’t wait to read the imaginative stories that come out of this unique contest,” said Nomi Dayan, Executive Director at The Whaling Museum.

Entries must be submitted electronically through the submission form on The Whaling Museum website by April 30, 2024. The contest is free to enter, and all works must be original and written solely by the author. Only residents of Nassau and Suffolk counties are eligible to participate.

“We look forward to the opportunity to celebrate and highlight the creative tales of students in the area,” said Daniel Dunaief, a journalist with TBR News Media. “Entrants can envision ways a piece of glass, shaped by water and time, provides a clue in a compelling narrative.”

Winners will be notified on or about June 1-7, 2024, and select winning entries will be eligible for publication in TBR News Media and on The Whaling Museum’s website. TBR will also highlight the winners in a weekly podcast. In addition, contest winners will receive complimentary tickets to The Whaling Museum’s Sea Glass Festival, held on July 21, 2024, where they will be recognized with a certificate.

“We are grateful to TBR News Media for partnering with us on this contest and helping to bring these young writers’ stories to a wider audience,” added Dayan.

For more information on the Sea Glass Fiction Contest, including guidelines and submission details, please visit cshwhalingmuseum.org/seaglasscontest.

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METRO photo

By Daniel Dunaief

When we are born, the experience is passive, as we don’t suddenly decide, despite what our families might tell us later about how we couldn’t wait to see the world, that it’s time to leave the womb.

Similarly, once we’re outside, we don’t make many choices. We can’t say, “Milk? Again? You don’t have orange juice or maybe a chocolate milkshake?”

The people around us, the customs that define our days and years, the languages we speak and many other factors that shape who we are remain outside our control.

Definitions of normal vary by our circumstances. People who share a single room and one bathroom with four siblings and those with four dogs, three cats, and two parakeets typically accept the conditions around them.

“Everyone has a crazy Uncle Allen and a chatty Aunt Dorothy,” they think. Or, perhaps, “everyone shops for a new wardrobe each year before the start of a school year” or “doesn’t everyone run three miles before breakfast every morning?”

And then, in the journey through life, we get a window into the lives of other people.

When our daughter was about four years old, she visited a friend, who, our daughter reported, drank soda with breakfast and ate candy as a post breakfast snack. “I like soda,” our daughter declared after the playdate. “Why can’t I have it with breakfast, too?”

It’s not just visits to other homes that become eye-opening experiences: we read about people, watch dramas about their lives, and get a sense of what we think we might strive for or reject in our expanding world.

We and our children see our families in a completely different light when we have the opportunity to compare them to the world outside. Sometimes, we not only measure up, but we exceed the limitations of other people’s lives. Our children might, for example, spend time with parents who pay little to no attention to their sons and daughters, barely aware of their comings and goings.

At that point, our helicopter parenting, which made them gnash their teeth every time we asked for more details about the events of their day, upcoming tests, school dances, or tryouts for school plays, might seem considerably less unbearable or even, dare I say it, charming.

Other times, we fall short in ways that even our children recognize is well outside the experience of most people. Some of their friends’ parents might own private jets, have a spare house on the lake, or have season tickets near the front row to watch one of their favorite teams.

Despite the id-driven desire to have similar life amenities, our children, sooner or later, recognize that they shouldn’t expect such lavish luxuries, even if they secretly, or, perhaps, not so secretly, hope to attain them.

And then there are the times when the world outside the family seems like the kind of easy-going, light-hearted, jovial tv show in which they’d like to star as the plucky but successful child.

During those moments, we can ask some questions about what they want or wish for that they don’t have, or that, perhaps, they find too cumbersome. Yes, we tell them, we really are related to that wacky Uncle Allen, but that doesn’t mean our children are going to become like him or that he has no redeeming qualities. Indeed, the search for redeeming qualities in everyone, starting with our own extended family, may help re-inflate our disappointed children.

If the head-to-head match up leaves them wanting something else, we have other options. We can suggest that no one will ever love or appreciate them as much as we do.

We can also suggest that they can use their desire for something more or different to inspire them to work hard for it and to find it for themselves and, if they choose to have one, for their own families.

Hopefully, our children can recognize that, on balance, the things that they considered givens — material, cultural, ethical and otherwise — didn’t drop from the sky, but came from hard work and the best of intentions.

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The Nova-C Class IM-1 Odysseus in preparation for launch. Photo courtesy of Wikipedia

By Daniel Dunaief

The stars aligned for a group of engineers and scientists in Washington D.C. recently, as a meeting brought these experts together at exactly the same time NASA was landing a vehicle on the moon for the first time since the finale of the Apollo missions, over 50 years ago.

The Nova-C Class IM-1 Odysseus in preparation for launch. Photo courtesy of Wikipedia

“I happened to be in DC for [NASA’s] annual Moon to Mars Architecture workshop on the day that the Intuitive Machines Odysseus spacecraft landed,” said Tim Glotch, Professor in the Department of Geosciences at Stony Brook University and the Science Chair of the Lunar Exploration Analysis Group, or LEAG. “The whole group I was with watched the live stream with excitement and nervousness.”

Attendees at the conference knew when NASA was supposed to receive a signal from the spacecraft. As they waited, Glotch said he could “feel people starting to think, ‘Uh oh, it started to happen again,’” raising the possibility of a problem with the landing. “A few minutes after the planned touchdown, everyone was relieved and overjoyed when the flight manager confirmed that they had a signal from the spacecraft,” he said.

Indeed, on February 22, the Odysseus lunar lander touched down about 185 miles from the moon’s south pole and within a mile of its target near the Malapert A crater. Glotch and the other scientists and engineers learned the next day at a press conference about some of the issues the spacecraft had when it landed, including the fact that it tipped over.

“From the standpoint of getting a soft touchdown on the moon in very challenging terrain near the south pole, this has to be considered a success,” he added.

Over the last few years, many attempts by companies and governments have demonstrated the challenges of landing on the moon, which is about 238,855 miles away, or the equivalent of over 9.5 times around the circumference of the Earth at the equator. Through NASA’s Commercial Lunar Payload Services program, private companies are trying to do what only a few governments had done, at considerably lower cost. 

“With every attempt, NASA’s commercial partners are learning the best ways to accomplish their goals of landing safely on the moon and successfully delivering NASA’s and other commercial partners’ payloads,” Glotch said. The Stony Brook professor described the overlap between the meeting and the landing as a “fun coincidence,” which created a “pretty big cheering section.”

Reflecting on the landing, Glotch shared his sense of pride in the space program, which is preparing to send people back to the moon through Artemis missions over the next five years.

“The fact that NASA was able to work with private corporations to develop the technology, not quite from scratch [but] to redevelop this capability is really impressive,” Glotch said. “It’s a great demonstration of ingenuity, determination and drive.” Engineers likely put in long days and nights making sure everything was ready, testing and retesting systems for this launch and landing, he said.

Lunar meeting

As for the gathering, Glotch said this second annual meeting provided an opportunity for scientists and engineers to discuss the future of travel to the moon and, potentially further in the future, a trip to Mars. 

The auditorium of the National Academies of Science, Engineering, and Medicine building where Tim Glotch and the other engineers and scientists were watching the landing attempt. Photo by Tim Glitch

A group of scientists and engineers are working together to learn to do the things on the moon that it will eventually do on the Red Planet. The team is focusing on a few bigger items that will matter in a relatively shorter term. After the meeting last year, Glotch said he “really got the sense that the architecture team [at NASA] had been listening to input from scientists.”

One of the bigger questions involves the amount of samples astronauts will bring back from the moon. Researchers expect some of these samples to contain volatiles like water and ice in them.

The scientific community has urged NASA to develop a plan to bring those rocks back frozen in their natural state. When the ice melts, it can cause chemical reactions to occur that make it more challenging to analyze them.

“If they are changing on the way back, we can’t be sure we’re getting the right answer” about where the water originated, he said.

Still, Glotch suggested that examining these defrosted rocks would provide considerable information.

NASA isn’t going to be able to keep the rocks under cold conditions for the first manned American mission that will return people to the Earth, aboard Artemis 3, which is scheduled to launch in September of 2026.

Starting with Artemis 5, in September of 2029, NASA, however, intends to include freezers to keep samples in pristine shape.

As the Science Chair for LEAG, Glotch brought up the need for a new lunar orbiter to characterize the surface at higher spatial resolution. The Lunar Reconnaissance Orbiter, which has been in space since 2009, has six or seven years of fuel left.

“Given that time frame, we need to develop a spacecraft and what type of instruments go on it,” said Glotch. NASA “really needs to think hard about funding a follow on orbiter.”

As for a manned journey to Mars, NASA plans to launch such a mission in the 2030s, according to the space agency’s website.

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Pixabay photo

By Daniel Dunaief

Daniel Dunaief

A long time ago, I joined a Freshman Outdoor Program trip before the start of college. The venture provided us with a chance to meet other incoming college students, to enjoy hiking, and to ask upper class students who were leading the effort questions about classes, places to eat on campus, and anything else that crossed our minds.

I was excited and anxious about my trek along the trail, in part because I was unaccustomed to relieving myself anywhere other than on a porcelain throne.

Recognizing my trepidation, my father, who sensed an opportunity to tease me, asked in the weeks before my trip how I was going to “poop in the woods.” My fear of taking care of business out in nature was even greater than my concern about my class selection, my choice of major or the unfamiliar roommates I would meet upon my arrival on campus.

In the days leading up to the trip, which lasted about a week, I tried to cut back on my food intake and I planned to use a bathroom with indoor plumbing as often as possible before climbing aboard the Appalachian-trail bound bus. For more than a day, I successfully shut my system down, avoiding the normal routine. Somehow, for close to two days, I managed to eat, carry about 60 pounds on my back, hike up and down mountains, and avoid pooping in the woods.

Then, as if my body refused to obey my stubborn will, I couldn’t take another step. Seeing me freeze on the trail, one of our upper class guides asked me what was wrong. Did my feet hurt? No. Did I need some water or food? No and no. Was I in pain? Yes, but not in the way I wanted to discuss.

I indicated that I had to “use the bathroom.” The guide told the group to stop, at which point I removed my backpack, took the small shovel we used to create our own buried fertilizer, and raced off to the left. How far, I wondered, would I have to go to avoid being seen by my fellow students, but be close enough that I didn’t seem like I was reading the New York Times while awaiting the arrival of the number two train?

With each step, my system recognized that I was getting closer to relieving itself, which meant that I couldn’t go much further without risking soiling myself. I picked a spot that had what looked like poison ivy. Moving over, I found another place that looked nothing like the comforts of home, but would have to do. After I dug a small hole, I squatted. I immediately felt something brush against my right butt cheek.

I turned around quickly and realized, with relief, that it was just a branch.

Throughout the decades that followed, I have put considerable effort into finding a toilet and to avoiding unpleasant restrooms. The search for a relatively clean and manageable bathroom has involved walking into nice hotels in cities around the country and world. 

To my great surprise, the McDonald’s at the Spanish Steps in Rome, which has a surprisingly appetizing-looking pasta bar that we couldn’t get ourselves to sample while in Italy, had remarkably clean bathrooms, which my wife and I used many times while trekking around the historic city.

Central Park, which is improbably spacious and beautiful amid the concrete jungle of Manhattan, has a web page with the locations of public restrooms around the park, although, despite living there for over a decade, I rarely ever used.

Throughout Manhattan, I have searched for restaurants, museums and bars, where the bathrooms don’t become stadium-level sullied until well after happy hour begins.

With the advent of social media, which took off well after I left New York City, I have found several pages dedicated to the process of finding a bathroom, including one called @poopersguide, which has pictures of the facilities.

Recently, I went to a fancier restaurant outside the city. To set the mood, the lighting was fairly dim. An accommodating waiter even came over, took out his iPhone and smoothly shined his flashlight over the menu.

When I excused myself to use the restroom, I came back with a small smirk on my face and was met with expectant looks.

“Well, that was the cleanest restaurant bathroom I’ve ever used,” I laughed. “I was a little concerned about using it. Oh, and I know where we can read the menu next time. The lighting in there was brighter than anywhere in my house.”

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Pixabay photo

By Daniel Dunaief

Daniel Dunaief

Even if you haven’t read the books, the way I did with my son when he was considerably younger, you probably have heard of the series “Diary of a Wimpy Kid,” right?

It occurred to me to ponder the possibility of a diary of an old(er) man. To that end, here’s my first installment and, no, I’m not going to start with “Dear Diary.”

I woke up this morning and I thought, hmm, my foot doesn’t hurt. What a delightful change! And then I stepped on the floor and I was wrong. My foot, which has been bothering me for a few weeks is still painful, despite the pleasant young doctor less than half my age examining it. The friendly technicians, who sent a jargon filled assessment to my electronic account, recognized that there was swelling and suggested a couple of possible options without a definitive diagnosis or conclusion.

Then, an assistant for my doctor called to schedule a time to review my results. She suggested he’d be available some time next week. Next week??? I gasped and tried not to become irrational or overwrought.

“But my foot hurts now. Is there anyone, like, maybe a nurse, who might be able to call me and tell me what to do for the pain or who can provide a first interpretation sooner than a week?”

I used the word “like” to sound younger, even as I was playing the older-man-in-pain card. Unfortunately, it didn’t teleport me to the front of the line.

“Oh, I’ll see what I can do,” she offered, transmitting a Mona Lisa-style tone through the phone line. Is that a smirk behind your voice? Is that the equivalent of a customer disservice line that says “we’re experiencing higher than normal call volumes and we’ll get to you when we can.”

The day passed without any calls from her, from a nurse, or from a doctor. Then, I thought about the people I used to be responsible for on an ongoing basis. How were they doing? Why hadn’t they called? Oh, right, they’re living their lives which is what my wife and I always wanted for them.

I hoped no news was good news, but no news is sometimes no news, until it makes a sudden transition to something that requires support and help or that merits cheerleading.

After reading through emails, I made some work calls. When reaching out to someone I didn’t know and leaving a message, I spelled my name, using the same “N” for Nancy, “E” for Edgar and “F” for Frank that I heard my father say so many times.

How much time in my life have I spent spelling my vowel-heavy name to someone? Realistically, the chance of the letters making it onto the paper in the right order or, more likely, into an electronic message is remote. More often than not, when someone says my name back to me, I say, “yes, that’s right,” even if they “dun arf” or “do vanoff.”

After I checked a few things off the personal and professional list, I scurried over to the gym, where people much younger than I lifted the weight of small Volkswagens, while others did the kind of abdominal exercises that I’d never attempted or considered. As I watched, trying not to let my jaw drop too far, my stomach hurt, signaling to my brain that I shouldn’t even think about trying those exercises … ever!

Later that night, my wife and I took a friend to a local sporting event, where it was cool enough to require a sweatshirt.

Following behind two people using canes as they walked, our friend asked if I thought a cane might help and, if so, whether he should take one from the men in front of us. I smiled and told him that wouldn’t be necessary.

As we approached our seats, a young woman said the two words associated with the name on the front of my sweatshirt, which advertised our son’s school. Three steps past her, I registered what she said and remembered what I was wearing. I considered turning around, but the moment had passed.

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A snail eating colubrid snake, Dipsas indica, from Peru.
Pascal Title with a semi-arborial colubrid snake, Chironius multiventris, from Peru.
A tree boar, Corallus hortulamus, which is an ambush predator, in Peru.
Pascal Title with a neotropical colubrine snake at the Los Amigos field station in Peru.
A false boa eating a lava lizard. Photo by Ivan Prates, University of Michigan
A CT scan of a cat-eyed snake that shows a frog (the blue skeleton) in its digestive tract. Photo by Ramon Nagesan, University of Michigan Museum of Zoology
An emerald tree boa in the amazonian rainforest, a specialized predator of small mammals. Photo by Dan Rabosky
an eyelash pitviper from the new world tropics. Photo by Alejandro Arteaga
Graham’s crayfish snake, a harmless semiaquatic species from central Noth America that feeds on crayfish. Photo by Tristan Schramer
A defensive display by a western ring necked snake native to the western United States. Photo by Alison Davis-Rabosky
A cat eyed snake in the Amazonian rainforest. Photo by Alison Davis-Rabosky

By Daniel Dunaief

Eating machines even more focused than teenagers approaching a stocked refrigerator, snakes slither towards foods other animals assiduously avoid.

In a recent and extensive study of snakes using the genetics, morphology and diet of snakes that included museums specimens and field observations, a team of scientists including Pascal Title, Assistant Professor in the Department of Ecology & Evolution at Stony Brook University, showed that the foods skin-shedding creatures eat as a whole is much broader than the prey other lizards consume.

At the same time, the range of an individual snake’s diet tends to be narrower, marking individual species as more specialized predators, a paper recently released for the cover of the high-profile journal Science revealed.

“If there is an animal that can be eaten, it’s likely that some snake, somewhere, has evolved the ability to eat it,” Dan Rabosky, senior author on the paper and curator at the Museum of Zoology and Professor of Ecology and Evolutionary Biology in the College of Literature, Science and the Arts at the University of Michigan, explained in a statement.

The research, which explored the genetics and diets of snakes, suggested that snakes evolved up to three times faster than lizards, with shifts in traits associated with feeding, locomotion and sensory processing.

“This speed of evolution has let them take advantage of new opportunities that other lizards could not,” Rabosky added. “Fundamentally, this study is about what makes an evolutionary winner.”

No singular physical feature or characteristic has enabled snakes to specialize on foods that are untouchable to other animals.

“It seems to be a whole suite of things” that allows snakes to pursue their prey, Title speculates.

One unique aspect of many advanced snakes is that they have more mobile elements in their skulls. Rock pythons can stretch their jaw around enormous prey, making it possible for them to swallow an entire antelope. Garter snakes, meanwhile, can eat Pacific newts that have a high concentration of a neurotoxin. Snakes also can eat slugs and snails that have evolved a defensive ability to secrete toxins.

A change to textbooks

Title, who is the co-lead and first author on the paper, suggested that the comprehensive analysis of snakes, particularly when compared with lizards, will likely change the information that enters textbooks.

“I think the analysis of lizard and snake diets in particular could potentially enter herpetology textbooks because diet is such a fundamental axis of natural history and because the visuals are so clear,” Title said. He doesn’t believe an analysis of dietary resolution that encompasses snakes and lizards has been shown like this before.

With a few exceptions, the majority of lizards eat terrestrial arthropods. Snakes have expanded into eating not only invertebrates, but also aquatic, terrestrial and flying vertebrates.

“They have absolutely evolved the ability to prey on semi-aquatic and aquatic prey,” said Title.

Title and his collaborators gathered considerable amounts of sequence data from GenBank. They also collected data from samples and specimens in the literature.

“Our dataset involves specimen-based data from museum collections that span the globe over the better part of the last century,” he explained.

The project started with the realization that several authors were generating high-quality sequence data for separate projects from biodiversity hotspots for lizards and snakes, such as in Australia, Brazil and Peru. The researchers realized that combining their data provided unprecedented coverage.

After Title completed his PhD at the University of Michigan, he took a leading role in building the phylogeny and conducting many of the analyses.

Indeed, the list of coauthors on this study includes 19 other scientists from the United States, the United Kingdom, Australia, Brazil and Finland.

As for his work, Title is broadly interested in the ecological/ environmental/ geographic/ evolutionary factors that lead to different species richness. He is not restricted to lizards and snakes.

“I do think snakes are unbelievable,” he said. “I’ve seen sidewinder rattlesnakes flip segments of their body forward across the sand in California, I’ve seen snakes climb straight up trees and walls, I’ve seen long, skinny snakes carefully navigate tree branches, and I’ve seen semi-aquatic snakes swim with their head above water. It’s mesmerizing.”

‘Snakes are cool’

Co-lead author Sonal Singhal, Assistant Professor in Biology at California State University, Dominguez Hills, met Title when she was a PhD student and he was an undergraduate at the University of California, Berkeley.

Singhal is excited that readers can “learn cool facts about snakes from our paper,” she explained. “Research papers don’t always inspire a sense of wonder in the reader.” She hopes people “walk away from this study thinking that snakes are cool.”

Singhal suggested that Title is leading a group of collaborators to create a package that will enable other researchers to download the data from this paper quickly and easily and use it in their own work.

As a whole, snakes are moving around in their diet space at a much more rapid clip than lizards in general, Title suggested.

While snakes have evolved rapidly over short periods of time, it’s unclear how these creatures are responding to changes in the environment on smaller time scales, such as through what’s currently occurring amid climate change.

The scale, Title explained, is different, with climate changes affecting the world over decades and centuries, while snake evolution, particularly regarding specialized diets, transpired over the course of millions of years.

Grad school encounter

Title, who lives in East Setauket, met his wife Tara Smiley when both of them were graduate students.

An Assistant Professor in the Department of Ecology & Evolution at Stony Brook University, Smiley is a paleoecologist specializing in small mammals.

The couple enjoys taking their son Micah, who is almost three years old, on camping trips and spending time outdoors.

As for the paper scoring the coveted spot on the cover of Science, Title suggested the exposure validates “that lizards and snakes, and their natural history, are inherently intriguing to all sorts of people, regardless of whether or not they are trained biologists.”

He hopes the work will not only inspire young scientists to learn more about snakes and lizards, but also to seek to quantify and explore the different axes of biodiversity and to “appreciate the value of supporting natural history museum collections.”

———————————————————————————–Within a day of snake research published on the cover of Science last week, reports surfaced about the discovery of what may be the largest snake in the world. Scientists from the University of Queensland found a northern green anaconda in the Ecuadorian Amazon that was close to 21 feet long.

Pacal Title, Assistant Professor in the Department of Ecology & Evolution at Stony Brook University and first author on the recent Science paper, offered his thoughts in an emailed question and answer exchange about the anaconda, which was not a part of his recent research.

TBR: Is this a particularly compelling find?

Title: This is compelling as it provides an example of broadly distributed, large species of snakes having pretty significant genetic differentiation. There are quite a few examples, both within snakes and in other groups, where populations look superficially similar, but turn out to have been genetically independent of one another for quite a long time.

TBR: How does a discovery of what might be the largest snake in the world fit into the context (if at all) of your research? Does this species validate the radiative speciation you described?

Title: It shows that the number of known snake species is likely to be an under-estimate, although this is likely to be true for most groups out there. This fits well into the perspective that snakes have incredibly high global species diversity.

TBR: Do you have any guesses as to what the diet of this snake could be?

Title: The article describes anaconda diets as generally consisting of terrestrial vertebrate prey, despite the species being semi-aquatic.

TBR: What, if any, predators might pursue this snake?

Title: Jaguars have been known to prey on anacondas.

TBR: What scientific, life history, genetic or other questions would you address, if any, about this species?

Title: Now that the green anaconda is being considered as two separate species, all morphological, ecological and natural history attributes will need to be re-examined to evaluate whether or not the two species actually differ along any of these axes.

TBR: Is the ongoing attention snakes receive positive for the study of snakes?

Title: It is great that snakes are receiving positive attention. Such new studies are essential for conservation, and for the study of biodiversity and ecosystems.

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From left, Prerana Shrestha, Sunghoon Kim (Postdoc), Andrew Gallagher (Research Support Specialist), Miura Traficante (SOAR Fellow, Summer undergrad researcher), Keith Yeung (Undergraduate researcher), Matthew Dickinson (PhD student), Saheed Lawal (PhD student), and Olivia Tabaka (MS student)

By Daniel Dunaief

An increasingly complex time filled with extreme stressors such as man-made and natural disasters creates conditions that can lead to post traumatic stress disorder.

PTSD, which can cause anxiety even amid safer conditions, can have adverse effects on the ability to enjoy life.

Prerana Shrestha

Stony Brook University Assistant Professor Prerana Shrestha, who joined the Department of Neurobiology and Behavior at the Renaissance School of Medicine in 2021, recently received a four-year $2.2 million grant from the National Institute of Mental Health to study the molecular mechanisms underlying stabilization of emotional memories in the brain, which is relevant for PTSD.

“Her work will help us understand how the brain stores these traumatic memories,” said Alfredo Fontanini, chair of the Department of Neurobiology and Behavior. “The tools she has developed really are making possible a series of experiments that, before, were impossible to think about.”

Shrestha hopes to develop a druggable target that could “block a key machine inside neurons that are relevant for traumatic emotional memories,” she explained.

Using a mouse model, Shrestha plans to understand the neural signature at the level of molecules, neurons, and neural circuits, exploring the creation and stabilization of these potentially problematic memories and emotional reactions through a multi-disciplinary study.

Shrestha has developed and applied chemogenetic tools to block a key part of the memory process inside neurons that store traumatic emotional memories.

By developing tools to explore neural circuits in particular areas of the brain, Shrestha can help scientists understand the molecular mechanism involved in PTSD, Fontanini said.

‘From the ground up’

In humans, memories from traumatic events are over consolidated, creating an excessive avoidance behavior that can be a debilitating symptom.

“We are trying to understand the neurological basis for why these memories are so robust,” Shrestha said. She is looking at “what can we do to understand the mechanism that supports these memories from the ground up.”

With her chemogenetic tools, Shrestha can block protein synthesis in specific neuron populations in a time period of a few hours. She is developing new tools to improve the precision of blocking the protein synthesis machinery from hours to minutes.

Shrestha is trying to weaken the salient emotional memory while leaving all other processes intact.

The Stony Brook Assistant Professor said she has methods to create a targeted approach that limits or minimizes any off target or collateral damage from inhibiting the synthesis of proteins.

“Up until now, whenever scientists wanted to study the role of the synthesis of new proteins in memory formation” including those involved in the formation of aberrant memories such as those in PTSD, they had to “use drugs which would manipulate and affect protein synthesis everywhere in the brain,” said Fontanini.

The plan over the next four years is to understand and develop molecules to target cells in the prefrontal cortex, which, Shrestha said, is like the “conductor of an orchestra,” providing top-down executive orders for the brain.

She is focusing on neurons that interact specifically with the amygdala, which is the emotional center of the brain, exploring what happens in these streams of information between brain regions.

By increasing or reducing protein synthesis in the prefrontal cortex, Shrestha can see an enhanced or diminished avoidance response in her mouse experiments.

She is interested in how a memory is stabilized, and not as much in what is involved in its retrieval.

Shrestha works with inbred mice that are more or less genetically identical. Her experimental group has the transgenic expression of the chemogenetic tool to block protein synthesis and receive a drug after learning that triggers the tool to block the machinery from making new proteins.

When she introduces the inhibitor of protein synthesis, she found that the wave involved in stabilizing what the animal previously learned is finite in time.

Using a drug to block protein synthesis within an hour alters future behavior, with the animal showing little or no fear. Blocking protein synthesis after that hour, however, doesn’t affect the fear response.

In the first year of the grant, which started in December, Shrestha would like to send out some papers for publication based on the research her team members — postdoctoral researcher  Sunghoon Kim and graduate student Matthew Dickinson —  has already done. She also hopes to use some of the funds from this grant to hire another postdoctoral researcher to join this effort.

She has data on how the regulators of ribosomes are recruited in the prefrontal cortex, which stabilizes memories.

In other preliminary data, she has identified neurons in the prefrontal cortex that project into the amygdala that are selectively storing information for recent parts of emotional memory.

To be sure, while this research offers a potential window into the mechanisms involved in forming emotional memories in a mouse model, it is an early step before even considering any new types of diagnostics or treatment for humans.

Nepal roots

Born and raised in Kathmandu, Nepal, Shrestha received a full scholarship to attend Bates College, in Maine, where she majored in biological chemistry. She received a Howard Hughes Medical Institute fellowship for an internship at Harvard Medical school during her junior year. While preparing for a pre-medical track, she “got spoiled after getting a taste of research in my junior year,” she said. “The idea of trying something new for the first time and seeing how things work was so cool.”

Shrestha lives about eight miles west of Stony Brook and is married to Sameer Maskey, the founder and CEO of an advanced machine learning company called FuseMachines Inc. They have a nine-year old daughter and a two-year-old son.

As for her ongoing work, Shrestha is eager to combine her expertise with those of people from different backgrounds.  “It’s a fascinating time to combine molecular approaches,” she said. 

Fontanini, who helped recruit Shrestha, has been impressed with the work she’s done.

“She’s on an outstanding trajectory,” he said.

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