CLOSE
Tags Posts tagged with "Daniel Dunaief"

Daniel Dunaief

by -
0 1952
Wellington Rody. Photo by John Griffin/Stony Brook University

By Daniel Dunaief

Straightening teeth involves moving, changing and reconfiguring the bone and the gums that hold those teeth in place. While the gums and bone adapt to the suddenly straight teeth, the roots may encounter unusual stress that makes them more prone to deterioration.

That’s not particularly welcome news for hormone-riddled teenagers who are maneuvering through the minefield of adolescence with a mouth full of metal. Fortunately, however, significant root damage that threatens the health and stability of teeth occurs in only about 5 percent of the cases of people with braces.

Wellington Rody. Photo by John Griffin/Stony Brook University

The challenge for people whose roots resorb in response to orthodonture is that most patients don’t show signs of problems until the process is well under way.

Wellington Rody Jr., the chair of the Department of Orthodontics and Pediatric Dentistry at the Stony Brook University School of Dental Medicine, hopes to change that.

Rody, who joined the staff at Stony Brook last May, received a $319,000 grant from the National Institute of Dental and Craniofacial Research to find biomarkers that may show early signs of periodontal disease and dental resorption.

Rody explained that he is “searching for noninvasive markers of root destruction.” Once orthodontists start moving teeth around, a major side effect can be that roots are compromised, to varying degrees. It’s a “common side effect with everybody that wears braces,” but it is usually minor with no clinical relevance.

Currently, the only way to discover root destruction from braces is through X-rays or CT images. “The problem is that when we find those things, as a clinical orthodontist, sometimes, it’s already too late,” Rody said.

Since biomarkers of bone destruction and root destruction may overlap, the focus of his research is to search for biomarkers that can differentiate between the two processes and find the markers that are more specific to root destruction. A few biomarkers of root destruction have been proposed, but there aren’t enough studies to validate those markers. 

Rody will be searching for markers in both saliva and gum fluid. He anticipates that a panel of biomarkers may be more successful than trying to focus on one marker only.

If the markers, which Rody has been developing in collaboration with Shannon Holliday, at the Department of Orthodontics at the University of Florida, and Luciana Shaddox, from the Department of Periodontology at the University of Kentucky, are effective, they will likely provide guidance to clinicians so that high-risk patients may have their treatment plan adapted to prevent further damage.

The type of molecules Rody is searching for include proteins, lipids, metabolites and RNAs. He has been using proteomics, but in this NIH grant, he received enough funding to extend the analysis to other molecules.

According to Rody, there are many predisposing factors for bone loss in the literature. Predisposing factors for root destruction in the dentition also exist but are not well validated.“Genetics definitely plays a major role,” but as far as he is concerned “there is not genetic testing that is 100 percent reliable.”

Until he discovers a reliable biomarker, Rody, who maintains a clinical practice at Stony Brook about one and a half days a week, suggests taking follow-up X-rays after initiating orthodontic treatment, to make sure the “roots are behaving properly,” he said.

A patient who develops serious root destruction may need active monitoring. If the resorption is severe enough, orthodontists typically recommend stopping treatment for a period of one to five months, which is called a “holiday,” and then resume treatment. 

Wellington Rody on vacation in California with his family. Photo from W. Rody

It is only recommended if the patient shows signs of moderate or severe root destruction. Another option is to interrupt treatment early and accept some compromises in the final results 

“We try to get the patient out of braces as soon as possible” in cases of severe root resorption, Rody explained.

Rody has been working in this area since 2014. He received initial funding from the American Association of Orthodontists Foundation. He started by simulating bone and root destruction in a lab and looking for different molecular signatures between the two processes and has already published articles that highlight these differences.

The current NIH study will allow for the search for potential biomarkers. If the group finds them, the next step would be to try to validate them through a process that is expensive and requires large trials.

Ultimately, if and when he finds those biomarkers, Rody said he can use them in a noninvasive way to closely monitor a patient with periodic X-rays. He also might adjust the treatment regime to make sure the patient receives positive results without compromising the prognosis for his or her teeth in the longer term.

Rody believes orthodontics are worth the risk of root resorption, as patients who develop this side effect will likely keep their teeth for many years if not for their whole lives, even with some reduction in their roots.

“Considering all the benefits that orthodontic treatment can bring, in terms of function and cosmetics, it’s still justifiable” but the patient and his or her parents need to understand the risks and benefits associated with braces, he said. 

The teeth that are typically affected by root resorption are the upper front teeth.

Originally from Vitória in Brazil, which is six hours by car north of Rio de Janeiro, Rody lives in Port Jefferson with his wife, Daniela, and their 14-year-old daughter, Thais. 

As for his research, Rody explained that a major goal is to “detect the process [of root resorption] before it becomes severe.” If he does, he will be able to “revise the course of treatment and make sure we don’t allow destruction” of roots and the potential consequences for teeth to reach a high level.

by -
0 1360
Stock photo

By Daniel Dunaief

Daniel Dunaief

We spend our lives searching. We look for friends in elementary school with whom we can share a laugh or a meal. We seek the right clothing and supplies so that we fit in.

As we age, the searches change. We hunt for fulfilling jobs, long-term romantic or career partners, places to live, cars that will meet our needs, and homes in communities that will welcome us and our families.

Through all of these searches, people wander into and out of our lives. If we’re fortunate enough, we might know someone from the time we’re 3 years old with whom we continue to meet, laugh, and exchange work stories or ideas and challenges.

Sitting in cars waiting for our children to emerge from their orchestra rehearsals or milling about in the entrance to an auditorium after a concert, we may see the same familiar faces, smile at the people next to us, and appreciate how they have supported all of our children with equal energy and commitment, congratulating our son or daughter on their solos or appreciating the remarkable live performance they just witnessed.

As we age, we inevitably lose people. Some drift out of our lives when their interests diverge from ours, even though they remain in the same town. Others take jobs in a new state and follow a different schedule in a new time zone.

When our friends or family members die, the losses are permanent. Except in photos, videos and in our imaginations, we won’t see their faces, smell their perfume or hear their infectious and distinctive laugh echo around a room.

We often say to family members and close friends, “So sorry for your loss.”

While death is a loss, it’s also a reminder of what we found. The person who has left us may have attended the same school, lived on the same block or gone to the same conference many years ago. A blur of people enter and leave our lives, sometimes for as short as a few seconds because we give them change at a store or take their reservations when we’re working for a ferry company, or other times when we’re waiting with them at the DMV to get a new license in a new state. Other times, the people who will become an ongoing part of our lives find us, just as we found them.

Their death brings sadness and a hole in the fabric of our lives. Some cultures tear a hole in their garments to tell the world about the missing piece that comes with mourning.

These moments are also an opportunity to celebrate the fact that we forged a connection and that we played an important role in each other’s lives.

Connections begin when we reach out to strangers who become friends and to men and women who become life partners. Every day, we have the opportunity to appreciate what we’ve found in the people who populate our lives, the ones we choose to call to share the news about a promotion, those whose support and consideration remind us of who we are.

When we stray from a path that works, these found friends can bring us back to the version of ourselves we strive to be. Each loss reminds us not only of who that person was in general, but also of what we discovered through our interactions. These important people provide common ground and experiences and are as much a part of who we are as the image staring back at us in the mirror. We didn’t just find them. Ideally, we found the best of ourselves through the experiences we shared with them.

by -
0 1204
Daniel Dunaief

When I was younger, I was the best baseball player who ever lived. OK, maybe that’s a wee bit of an exaggeration. Maybe I was a decent player who had a few good games, surrounded by periods of agonizing ineffectiveness, miserable failure and frustrating inadequacies.

Baseball, as its numerous fans will suggest regularly, is a game of failure. And yet those exquisite moments of success — when we break up a no-hitter, get to a ball that seemed destined for open grass or develop the speed to outrun the laser throw from the outfield — make us feel as if we can do anything.

Recently, I have found myself frustrated beyond the normal measure of perspective because I feel as if I’ve lost a step or six when I play softball. My current athletic deficiencies seem to be a harsh reminder of the inexorable journey through time.

As I return from the game in the car, I sometimes bark questions at myself, wondering how I missed an easy pop-up, or how I lunged for yet another pitch I should have hit. My family, who comes to the games to support me, watches me dissolve into a puddle of self-loathing.

Yes, I know, it’s not my finest hours as a parent and I know I’m setting a terrible example. And yet something inside of me, which is both young and old, can’t control the frustration. I’m an older version of the kid who was so annoyed with his own deficiencies that he kicked a basketball over some trees. OK, maybe they were hedges and I probably threw the ball, but in my memory the offending orb traveled a great distance.

So, what was and sometimes is missing from my life that caused these games to be so important? Other than talent, conditioning, plenty of sleep and a commitment to practicing, my biggest problem was, and sometimes still is, a lack of perspective.

People suffer through much greater hardships than a decline in limited athletic skills. Life is filled with challenges and inspiration. People overcome insurmountable odds, push themselves far beyond any expectations by taking small steps for mankind or even small steps for themselves when they weren’t expected to walk at all.

As I know, I am fortunate in many ways to have the opportunity and time to play softball at all. To be sure, I recognize that perspective isn’t what people generally need when they care about something large or small: They need focus. Artists spending countless hours painting, writing, revising, editing or reshooting a scene for a movie to enable the reality of their art to catch up to their vision or imagination often lose themselves in their efforts, forgetting to eat, to call their parents or siblings, to sleep or to take care of other basic needs.

Considerable perspective could prevent them from finding another gear or producing their best work.

And yet perspective, particularly in a moment like a softball game, can soothe the escalated competitor and give the father driving a car with his supportive family a chance to appreciate the people around him and laugh about his inadequacies, rather than dwell on them.

In a movie, perspective often comes from a camera that climbs high into the sky or from someone looking through a window at his children playing in a yard or at a picture of his family in a rickety rowboat. Perhaps if we find ourselves tumbling down the staircase of anger, frustration or resentment, we can imagine handrails we can grab that allow us to appreciate what we have and that offer another way of reacting to life.

by -
0 2062
Maureen O’Leary wraps fossils during an expedition in Mali. Photo by Eric Roberts

By Daniel Dunaief

Mali is filled with challenges, from its scorching hot 125 degree temperatures, to its sudden rainstorms, to its dangers from militant and terrorist-sponsored groups.

The current environment in the landlocked country in West Africa makes it extraordinarily difficult to explore the past in a region that includes parts of the Sahara Desert, but that, at one point millions of years ago, was part of a waterway called the Trans-Saharan Seaway.

Maureen O’Leary, professor of anatomical sciences at the Renaissance School of Medicine at Stony Brook University, led three expeditions to Mali, in 1999, 2003 and 2008, collecting a wide array of fossils and geological samples from areas that transitioned from an inland seaway that was about 50 meters deep on average to its current condition as a desiccated desert.

Maureen O’Leary and Eric Roberts with Mali guards. Photo from Maureen O’Leary

On her third trip, O’Leary quickly left because she decided the trip was too dangerous for her and the scientific team. Rather than rue the lack of ongoing access to the region, however, O’Leary pulled together an international team of researchers from Australia, the United States and Mali to look more closely and categorize the information the research teams had already collected from the region.

“We made the most of a bad situation,” O’Leary said. “It is a silver lining, to some degree.”

Indeed, O’Leary and her collaborators put together a paper for the June 28 issue of the Bulletin of the American Museum of Natural History that is over 170 pages and contains numerous images of fossils, as well as recreations of a compelling region during a period from 100 million to 50 million years ago. This time period coincided with one of the five great prehistoric extinction events, during the Cretaceous-Paleogene boundary.

O’Leary characterized some of the more exciting fossil finds from the region, which include the first reconstruction of ancient elephant relatives and large predators such as sharks, crocodiles and sea snakes.

The size of some of these creatures far exceeds their modern relatives. For example, O’Leary’s scientific colleagues estimate that a freshwater catfish was about 160 centimeters in length, which is four times the total size of a modern catfish. The larger catfish dovetails with similar observations the researchers had made about sea snakes in 2016 and 2017. They started to knit this trend into a preliminary hypothesis in which a phenomenon known as island gigantism may have played a role in selecting for these unusually large creatures.

“Species become bigger in these environments,” O’Leary said, suggesting that other scientists have made similar observations. “It’s not clear what causes that kind of selection.”

Above, some of the species that lived in and around the TransSaharan Seaway, including an extinct species of crocodile. Illustrated by Lucille Betti-Nash/ Department of Anatomical Sciences, Stony Brook University.

 

In addition to studying vertebrate and invertebrate fossils, scientists including Eric Roberts at James Cook University in Australia looked at the geology of the region. Roberts helped name and describe many of the formations in the area. This provides context for the lives of creatures who survived in an environment distinctly different from the modern milieu of the Sahara Desert.

Roberts, who is a part of the Sedimentary Geology & Paleontology Research Group that has nicknamed themselves Gravelmonkeys, explained that his initial efforts in Mali came from the fieldwork over a course of weeks when he explored the rock sequences and took copious notes on them.

He suggested that the region still represents a geoscience frontier, in part because it is so difficult to get to, takes serious logistics to do fieldwork and is hard to maintain research.“Over many years, I have worked with collaborators on the project to analyze the samples in many different ways and especially to compare our notes and analytical results with descriptions of rocks and geological formations in other parts of the Sahara and further afield in Africa to understand how they are different and how they correlate,” he said.

O’Leary suggested that the paper provides some context for climate and sea level changes that can and have occurred. During the period she studied, the Earth was considerably warmer, with over 40 percent of today’s exposed land covered by water. Sea levels were about 300 meters higher than current levels, although the Earth wasn’t home to billions of humans yet or to many of the modern day species that share the planet’s resources.

Robert Voss, the editor-in-chief of the series at the American Museum of Natural History, praised the work for its breadth. “This was an unusually large and multidisciplinary author team, as appropriate for the broad scope of the report,” he explained .

“Seldom is such a large geographic area so poorly known paleontologically, so there was a unique opportunity here to break new ground and establish a broad framework for future work,” he added.

Voss described O’Leary as a “force of nature” who “responds constructively to peer reviews.” Roberts, too, appreciated the effort O’Leary put into this work.

O’Leary “drove the entire process and product,” which was only possible with someone of her “vision to wrangle so much science from so many different scientists into one place,” he offered in an email.

Roberts is very pleased with the finished product and added that it is “something that I will be proud of for the rest of my career. This took a lot of effort over the years and it great to see the end product.”

O’Leary said that much of the literature for the science in Mali was in French, which had kept it a bit below the radar for scientific discourse, which tends to be in English.

Indeed, O’Leary was able to facilitate conversations among the many people involved in this project because French was the common denominator language. She studied French at the Holton-Arms School in Bethesda, Maryland. “When I was sitting in my high school French class, I didn’t think it would come in so handy to be fluent in French” in her career, O’Leary said. “It was helpful as a female leader in this situation to be able to speak for [myself], whether speaking to other Americans or collaborating or working with guards.”

O’Leary plans to look at different projects in the United States, including in Puerto Rico, and in Saudi Arabia next. “We now have this synthetic story for Mali [and will be] building out from this to other areas. I anticipate a large time to ramp up to study areas like deposits in Nevada.”

by -
0 1795
Gábor Balázsi. Photo from SBU

By Daniel Dunaief

Take two identical twins with the same builds, skill sets and determination. One of them may become a multimillionaire, a household name and the face of a franchise, while the other may toil away at the sport for a few years until deciding to pursue other interests.

What causes the paths of these two potential megastars to diverge?

Gábor Balázsi, an associate professor in biomedical engineering at Stony Brook University, asked a similar question about a cellular circuit in the hopes of learning more about cancer. He wanted to know what is it about the heterogeneity of a cancer cell that makes one susceptible to treatment from chemotherapeutic drugs and the other resistant to them. Heterogeneity comes from molecular differences where the original causes may be subtle, such as two molecules colliding or a cell being closer to the tumor’s surface, while the consequences can create significant differences, even among cells with the same genes.

In research published this week in the journal Nature Communications, Balázsi used two mammalian cell lines that were identical except that each carried a different synthetic gene circuit that made one more heterogeneous than the other. He subjected the two cell lines, which would otherwise perform the same function, to various levels of the same drug to determine what might cause one to be treatable and the other to become resistant. 

Through these mammalian cells, Balázsi created two circuits, one of which kept the differences between the cells low, while the other caused larger differences. Once inserted in the cell, these gene circuits created uniform and variable populations that could serve as models for low and high heterogeneity in cancer.

Working with Kevin Farquhar, who recently graduated from Balázsi’s lab, and former Stony Brook postdoc Daniel Charlebois, who is currently at the Department of Physics at the University of Alberta, Balázsi tried to test how uniform versus heterogeneous cell populations respond to treatment with different drug levels. 

Using the two synthetic gene circuits in separate but identical cell lines, the Stony Brook scientists, with financial support from the National Institutes of Health and the Laufer Center for Physical and Quantitative Biology at SBU, could re-create high and low stochasticity, or noise, in drug resistance in two cell lines that were otherwise identical.

While the work is in its preliminary stages and is a long way from the complicated collection of genes responsible for various types of cancer, this kind of analysis can test the importance of specific processes for drug resistance.

“Only in the last decade or so have we come to realize how much heterogeneity (genetic and nongenetic differences) can exist within a tumor in a single patient,” Patricia Thompson-Carino, a professor in the Department of Pathology at the Renaissance School of Medicine at SBU, explained in an email. “Thinking of cancer in a single patient as several different diseases is a bit daunting, though currently, this heterogeneity and its direct effects on how the cancer behaves remains poorly understood.”

Indeed, Thompson-Carino added that she believes Balázsi’s work will “shed light on cancer cell responses to therapy. With the rise in cancer therapies designed to specific targets and the resistance that emerges in patients on these therapies, I think [Balázsi’s] work is of extremely high value” which may help with the puzzle of how nongenetic or epigenetic heterogeneity affects responses to treatment, she continued.

In the future, researchers and clinicians may look to develop new ways of biomarker analysis that considers the variability, rather than just the average level of a biomarker.

Balázsi suggested that looking only at the variability of cells is analogous to observing an iron block sinking in water. Someone might conclude that all solids sink in liquids. Similarly, scientists might decide that cellular variability always promotes drug resistance from observations when this happens. To gain a fuller understanding of the effect of variability, however, researchers need to equalize the averages. They then need to explore what happens at various levels of drug treatment.

Current therapies do not target heterogeneity. If such future treatments existed, doctors and scientists could combine ways of treating heterogeneity with attacking cancer, which might work in the short term or prevent cancer from recurring.

Balázsi suggests his paper is a part of his attempt to address three different areas. First, he’d like to figure out how to categorize patients better, including the variability of biomarkers. Second, he believes this kind of analysis will assist in creating future combinations of treatments. By understanding how the variability of cancer cells contributes to its reaction to therapies, he might help create a cocktail of treatments, akin to the effort that helped with the treatment of HIV in the lab.

Third, he’d like to obtain cancer samples and allow them to evolve in a lab, where he can check to see how they respond to treatment levels and administration scheduling. This effort could allow him to determine the optimal drug combination and dosing for a patient.

For the work that led to the current Nature Communications paper, Balázsi explored how mammalian cells respond to various concentrations of a drug. Over 80 percent of the genes in these cells are also present in human cells, so the mechanisms he discovered and conclusions he draws should apply to human cancer cells as well.

He concluded that cells with more heterogeneity, where the cells deviate more from the average, resist drugs better when the drug level is high. These same cells, show greater sensitivity when the drug is low.

Balázsi recognizes that the work he’s exploring is a “complex problem” and that it requires considerable additional research to understand and appreciate how a therapy might kill one cancer cell, while the same treatment in the same environment doesn’t have the same effect on a genetically identical cell.

by -
0 1419

By Daniel Dunaief

Daniel Dunaief

We make them before we even get up. We lie in our beds, staring at our alarm clocks, where we are faced with the first of countless decisions. Should we get up now or can we afford to wait a few minutes before climbing out of bed?

Decisions range from the mundane to the mind blowing: Do you want pickles, lettuce and tomatoes and what kind of bread would you like; you’re taking a pay cut so you can do what job exactly; are you sure you want to sell that stock today when it may be worth more tomorrow?

We rarely take a step back from the decision-making process because we generally don’t want to slow our lives down, leaving us less time to make other decisions.

Some of the decisions we make are through a force of habit. We buy the same ketchup, take the same route to work, wear the same tie with the same shirt or call the same person when we are feeling lonely.

Just because we have always done something one particular way, however, doesn’t mean we made the best choice, or that we considered how the variables in our lives have changed over time.

As we age, we find that our needs, tastes and preferences evolve. Our bodies may have a lower caloric demand, especially if we spend hours behind a desk. We might also be more prepared to debate or argue with our priest or rabbi, or we might have a greater need to help strangers or make the world a better place for the next generation. The way we make decisions today may be inconsistent with the way we made them for the younger versions of ourselves.

We may have some of the same tastes for movies or books that we had 20 years ago. Then again, we may place a higher value on experiences than we do on possessions.

Eating a particular food, calling a person who makes us feel inadequate or sticking with the same assignments or jobs is often not the best way to live or enjoy our lives.

Inertia affects the way we decide on anything from whether to vote Democratic or Republican to whether we would like pasta or salad for lunch. Sure, I could defy the old me. But then am I remaking a decision or remaking myself?

Ah, but there’s the real opportunity: We can follow the Latin phrase “carpe diem” — seize the day — and redefine and reinvent ourselves as long as we do it with purpose and focus.

Sure, that takes work and planning and we might change something for the worse, but maybe we would make our lives better or leave our comfort zone for greater opportunities. We can decide to take calculated risks with our lives or to move in a new direction. After all, we teach our children to believe in themselves. And if we want to practice what we preach, we should believe in ourselves, too, even on a new path.

Why should we put our lives on automatic pilot and sit in the back seat, making the same circles month after month and year after year? Some routines and decisions, of course, are optimal, so changing them just to change won’t likely improve our lives.

But for many decisions, we can and should consider climbing back into the driver’s seat. For a moment, we might cause our paths to rock back and forth, as if we shook the wheel, but ultimately we can and will discover new terrain.

by -
0 1738
Ela Elyada. Photo by Giulia Biffi

By Daniel Dunaief

They have the ability to call the body’s armed forces. They may interact with the immunological foot soldiers and, then, somehow, inactivate them, allowing the destructive cancer they may aid and abet to continue causing havoc.

This is one hypothesis about how a newly discovered class of fibroblasts may play a role in the progression of pancreatic cancer.

Ela Elyada, a postdoctoral fellow in David Tuveson’s lab at Cold Spring Harbor Lab, partnered up with Associate Professor Paul Robson at the Jackson Laboratory in Farmington, Connecticut, to find a new class of fibroblast in pancreatic cancer.

This cell, which they called antigen-presenting cancer-associated fibroblasts (or apCAFs) had the same kind of genes that are usually found in immune cells. Cells with these genes have signals on their surface that present antigens, or foreign parts of viruses and bacteria to helper T-cells. Elyada and Robson showed that the apCAFs can use their immune cell genes to present peptides to helper T-cells.

With the apCAFs, the researchers hypothesize that something about the immunological process goes awry, as the T-cells show up but don’t engage.

Elyada and Robson suspect that the activation process may be incomplete, which prevents the body’s own defense system from recognizing and attacking the unwelcome cancer cells.

While she was excited about the potential of finding a different type of cell, Elyada needed to convince herself, and the rest of the scientific community, that what she’d found was truly original, as opposed to a scientific mirage.

“We spent hours and hours trying to understand what is different in this type of cell,” Elyada said. “Like everything new you find, as a scientist, you really question yourself, ‘Is it real? Is it an artifact of the single cell?’ It was really important for me to do everything I could from every angle to make sure they were not macrophages that looked like fibroblasts or cancer cells that looked like fibroblasts.”

After considerable effort, Elyada was sure without a doubt that the group had found fibroblasts and that these specific cells, which typically are involved in connective tissue but which pancreatic cancer uses to form a shell around it, contained these immunological genes.

She sees these cells in different experiments from other people inside and outside the lab, which further supports her work and found the apCAFs in mice and human pancreatic ductal adenocarcinoma, which is the fourth leading cause of cancer-related deaths in the world.

The fibroblasts, which are not cancerous, play an unclear role in pancreatic cancer. 

Elyada explained that single-cell sequencing enables scientists to look at individual cells, instead of at a whole population of cells. Scientists “have started to utilize this method to look at differences between cells we thought were the same,” she said. “It’s useful for looking at the fibroblast population. Scientists have appreciated that there’s probably a lot of heterogeneity,” but they hadn’t been able to describe or define it as well without this technique.

The results of this research, which was a collaboration between Elyada, Robson and others, were recently published in the journal Cancer Discovery. Robson said it was a “great example of how [single-cell RNA sequencing] can be very useful in revealing new biology, in this case, a new subtype of cancer-associated fibroblast.”

Earlier work in the labs of Robson and Tuveson, among others, have shown heterogeneity within cancer-associated fibroblast populations. These often carry a worse prognosis.

“We are very interested in continuing to explore this heterogeneity across tumor types and expect we will continue to find new subtypes and, although we have yet to confirm, would expect to see other solid tumor types to contain apCAFs,” Robson said.

“We still need to work hard to reveal their function in the full animal, but if they turn out to be tricking the immune cells, they could be a target for different immune-related inhibition methods,” explained Elyada.

The newly described fibroblast cells may be sending a signal to the T-cells and then either trapping or deactivating them. Elyada and Robson both said these results, which they developed after working together since 2016, have led to numerous other questions. They want to know how they work, what the mechanisms are that allow their formation, what signals they trigger in T-cells and many other questions.

Elyada is working with Pasquale Laise in Andrea Califano’s lab at Columbia University to gather additional information that uses this single-cell sequencing data.

Laise has “a unique way of analyzing [the information] to look at how the sequencing can predict if proteins are active or not active in a cell,” she said. Laise is able to predict the activity of transcription factors according to the expression level of their known target.

Elyada may be able to use this information to understand the source cell from which the fibroblasts are coming.

Originally from Israel, Elyada has been working as a postdoctoral researcher in Tuveson’s lab for about six years. She lives in Huntington Village with her husband Gal Nechooshtan, a postdoctoral researcher at Cold Spring Harbor Laboratory’s Woodbury complex. The couple has two daughters, Maayan, who is 10, and Yael, who is 8.

Elyada hopes to return to Israel next year, where she’d like to secure a job as a professor and build on the work she’s done at CSHL.“I definitely want to keep working on this. This would hopefully be a successful project in my future lab.”

by -
0 1350
An Italian immigrant family on board a ferry from the docks to Ellis Island, New York. (Photo by Lewis W Hine/Getty Images)

By Daniel Dunaief

Daniel Dunaief

We cry and laugh with movie characters, feeling their pain when their fictional lover runs away with the neighbor or laughing with them when they share a joke, slip and fall, or embarrass themselves during a public speech.

Long after we’ve put a book down, the characters join us as we commute back and forth to work. We feel the pain they experienced during World War II when they lost family members or neighbors. We are grateful that the main character who is battling his personal demons somehow survives unimaginable ordeals.

We stare into the faces of the huddled masses from pictures at Ellis Island, many of whom left the only home they’d ever known to start a new life in a place that has become, fortunately for so many of us, the only home we’ve ever known. We see the bags at the immigrants’ sides, the children in their arms who are our parents and grandparents, and the resolve in the arrivals’ eyes as they wait for their turn to pass through the gates to the New World.

We read about people whose lives touch us so profoundly that we send money through GoFundMe pages. We don’t have any need to ask them whether they drink Coke or Pepsi, whether they’re a Democrat or a Republican, or if they support France or the United States in the Women’s World Cup. We want something better for them.

What about all the people who surround us, who drive next to us on the same roads on the way to and from work, who stand in line with us at the movie theater, the deli or the Department of Motor Vehicles?

The people who share time and place with us are just as deserving of our sympathy, empathy and care, and yet we honk when the light turns green and they don’t go, we become irritated when they don’t understand our lunch order, and we snarl when our co-workers misunderstand an assignment.

I would like to suggest that we spend one day every year, maybe this publication day, June 27, appreciating people. Let’s call it People Appreciation Day.

This doesn’t and shouldn’t be a day when we trudge out to get a mass produced card that says, “Hey, I appreciate you.” This could be any level of appreciation we’d like to share.

We could take an extra second to thank the cashier at the supermarket, who asks us for our store card and wants to know if we found everything OK. We can thank her and ask how she’s doing. When she answers, we might react accordingly: “Oh, happy birthday” or “Sorry to hear about your cat” or “I sometimes miss the place where I grew up, too.”

Maybe instead of honking when the light turns green, we can imagine — the way we would if we were looking at the title of a movie or the cover of a book — what the driver inside is feeling, thinking or experiencing. How is that any different from caring about a two-dimensional stranger in a book we’re holding?

The people in our lives aren’t here to entertain or amuse us, but they can elicit our empathy, understanding and appreciation. We can, however, offer them the gift of care and concern.

We can appreciate their efforts to meet their basic needs and their desire to strive for something better for themselves and their children. These other people are dedicated teachers, determined athletes, a third-generation member of the military or a new neighbor from far away whose loneliness we can extinguish. Let’s take the time and put out the effort to appreciate them. When we do, we can benefit from the opportunity for people appreciation to forge a human connection.

by -
0 2025
Mircea Cotlet. Photo courtesy of BNL

By Daniel Dunaief

An innovative scientist in the world of nanostructures, Mircea Cotlet recently scored Inventor of the Year honors from Battelle.

A principal investigator and materials scientist in the Soft and Bio Nanomaterials Group at the Center for Functional Nanomaterials at Brookhaven National Laboratory, Cotlet has conducted a wide range of research over his dozen years on Long Island.

The distinction from Battelle, which manages BNL through Brookhaven Sciences Associates, honors researchers who have made significant scientific or engineering contributions that have societal or financial impacts.

“The award recognizes [Cotlet’s] ongoing contributions to materials science at BNL, specifically his work on low-dimensional semiconductors, 1-D nanowires, and tiny 0-D nanocrystals called quantum dots,” Katy Delaney, a Battelle spokesperson, explained in an email.

Researchers who have worked with Cotlet believe he deserves the honor.

Cotlet is an “extraordinary scientist” who “stands out” for his thorough work and creative approach” said Deep Jariwala, an assistant professor in the Department of Electrical and Systems Engineering at the University of Pennsylvania. Jariwala has known Cotlet for over two years and has collaborated with him over the last year.

Cotlet has “really laid the foundational ground in understanding the rules that govern charge and energy transfer across hybrid quantum confined materials systems that comprise quantum dots, organic molecules–two-dimensional materials as well as biologically photoactive materials,” Jariwala added.

The technologies will impact the science and technologies of sensing, displays and energy harvesting in the future, Jariwala predicted.

Eric Stach, a professor in the Department of Materials Science and Engineering at the University of Pennsylvania who had previously worked at the CFN, said Cotlet “tries to figure out ways of putting together disparate systems at the nanoscale.”

By combining these materials, Cotlet is able to “improve the overall performance” of systems, Stach continued. “He’s trying to tune the ability of a given material system to capture light and do something with it.”

Cotlet recently partnered self-assembled two-dimensional nanoparticles, such as the one-atom-thick graphene, with light-absorbing materials like organic compounds.

The result enhances their ability to detect light, which could be valuable in medical imaging, radiation detection and surveillance applications. The mini-partnership boosted the photoresponse of graphene by up to 600 percent by changing the structure of the polymer.

Indeed, a defense contractor has shown an interest in research they could use for low light level detection applications, Cotlet said.

Like other scientists at BNL, Cotlet not only conducts his own research, but he also helps other scientists who come to the Department of Energy facility to use the equipment at the CFN, to make basic and translational science discoveries.

Cotlet patented a self-assembly process before he published it.

He is continuing conversations with a big company that is exploring the benefits of this type of approach for one of its product, while he is also working with the technology transfer office at BNL to look at the development of photodetectors for low light applications.

“Having graphene and the conductor polymer would absorb light from ultraviolet to visible light,” Cotlet said.

The physics changes from bulk to nanoparticles to nanocrystals, Cotlet said, and he engineers the smaller materials for a given function.

“We basically like to play with the interface between different types of nanomaterials,” he said. “We like to control the light-simulated process.”

Working at an energy department site, he also has experience with solar panels and with light-emitting diodes.

Jariwala described the science as extending to interfaces that also occur in nature, such as in photosynthesis and bioluminescence. “By combining techniques and materials that we have developed and looked at, we hope to answer fundamental mechanistic questions and provide insights into long-standing questions about biological energy conversion processes,” he wrote.

As far as some of the current materials he uses, Cotlet works on graphene and the transition metal dichalcogenides and he explores their potential application as quantum materials. He tries to look for emerging properties coming out of nanomaterials for various applications, but most of his efforts are in basic science.

Jariwala explained that he and Cotlet are seeking to understand the efficient transduction of energy in quantum sized systems when they are brought close to one another in an orderly fashion.

After his upbringing in Romania, where he attended college, Cotlet appreciated the opportunity to learn from one of the pioneering groups in the world in single-molecule microscopy at the Katholieke Universiteit Leuven in Belgium, where he studied for his doctorate.

He also did a fellowship at Harvard, where he worked on unique microscopy, and then went on to conduct postdoctoral work at Los Alamos National Laboratory, where he worked on protein folding and on optimal imaging methods.

Cotlet arrived at the CFN just as the facility was going online.

“The CFN went beyond its original promise for cutting edge science,” he said. The center has been, and he continues to hope it will be, the best place he could dream of to conduct research.

The postdoctoral researchers who have come through his lab have all been successful, either leading their own projects or joining commercial teams.

Up until he was 18, Cotlet wasn’t focused on science, but, rather, anticipated becoming a fighter pilot. He discovered, however, that he had a vision defect.

“All my childhood, I was set up to become a fighter pilot,” but the discovery of a condition called chromatopsy changed his plans.

A resident of Rocky Point, Cotlet lives with his wife, Ana Popovici, who is an administrative assistant at BNL, and their middle school daughter.

As for his future work, he is interested in building on the research into quantum materials.

“I’m looking forward to trying to integrate my research” into this arena, he said.

by -
0 1069
Stock photo

By Daniel Dunaief

Daniel Dunaief

Breaking up is spectacularly awkward, highly charged and, in retrospect, filled with humorous potential. Two people get together for a picnic, where a public scene might be difficult for the recipient.

“Want some tabouli? What is tabouli anyway?”

“No thanks, and I don’t know what it is. You ordered it, not me.”

“Good point, so, I was thinking. It’s probably a good time for us to separate.”

“Um, what, excuse me?”

The lip quivers, the breathing becomes short and erratic and the eyebrows, shoulders and neck all droop at the same time.

“No, yeah, I mean, you’re great and this has been a total blast but, you know, it’s just, I don’t know, it’s not working for me.”

“A total blast? You’d call this a total blast? Besides, nothing is perfect. I know my family can be difficult and I know I wake up with bad breath and I do, on occasion, correct your speech, but we can work around that. Don’t you want to try to make it work?”

“I’m thinking that it’s probably time to do other things. I’m thinking of moving to Vancouver and you hate the cold.”

“Vancouver? Really? Wait, have you been seeing other people? You and my sister get along a little too well. As soon as you start dating her, she won’t be interested. I know I share genes with her, but she’s a horrible person who has ruined my life over and over again.”

“No, really, this has nothing to do with your sister. I wouldn’t do that to you or myself, especially after what you just said.”

“Oh, so, now there’s something wrong with my sister? At least she’s not dumping me.”

“No, no, I think we have a great friendship and I’d like to stay in touch.”

“You’d like to stay in touch? After all we’ve been through, you’re offering me your friendship? You’re not even that good of a friend. You rarely listen and you forget all the important dates in the year and you always want to go to the same restaurants, even though we have so many other choices.”

“Right, exactly, I’m so boring, so maybe you’re ready to be done with me?”

“Why do we have to end it now? It’s not like I was expecting to marry you. I can’t imagine having a younger version of you in the house. You can somehow shoot baskets from all over a gym floor that land in a hoop, but you have no ability to throw the dirty T-shirt you wore to play basketball into a much larger hamper that’s also closer to the ground, even though you roll the shirt into a ball.”

“I agree. You could do so much better.”

“I’m sure there are plenty of better people out there, but we had some fun, right? We were supposed to go to that dinner next Saturday with the Smiths. They’re your friends, so maybe we should see what works between now and then?”

“It’s OK, I already canceled that.”

“What? That horrible person Jessica Smith knew you were going to break up with me before I did? How could you do this to me?”

“Sorry, I didn’t tell them anything. I just said we couldn’t make it.”

“We couldn’t make it because you were going to break up with me today over tabouli. You’re an idiot.”

“Right, well, maybe we shouldn’t stay in touch?”

“Oh, so now I’m not good enough to be your friend?”

“I’m going to be a boring idiot elsewhere.”

“Wait, you’re leaving me?”

“Yes, and I’ve googled ‘tabouli.’ It’s a Lebanese salad with vegetables, wheat and parsley, just so you know.”

Prev1...686970...88Next Page 69 of 88
TBR News Media is your local news and entertainment website.
We provide you with the latest breaking news and information for your community.
Contact us: [email protected]

Most Viewed

©